4FI3: Structure Of Vitamin B12 Transporter Btucd-F In A Nucleotide-Bound State

Citation:
Abstract
The ATP-binding cassette (ABC) transporter BtuCD mediates the uptake of vitamin B(12) across the inner membrane of Escherichia coli. Previous structures have shown the conformations of apo states, but the transport mechanism has remained unclear. Here we report the 3.5 A crystal structure of the transporter-binding protein complex BtuCD-BtuF (BtuCD-F) trapped in an beta-gamma-imidoadenosine 5'-phosphate (AMP-PNP)-bound intermediate state. Although the ABC domains (BtuD subunits) form the expected closed sandwich dimer, the membrane-spanning BtuC subunits adopt a new conformation, with the central translocation pathway sealed by a previously unrecognized cytoplasmic gate. A fully enclosed cavity is thus formed approximately halfway across the membrane. It is large enough to accommodate a vitamin B(12) molecule, and radioligand trapping showed that liposome-reconstituted BtuCD-F indeed contains bound B(12) in the presence of AMP-PNP. In combination with engineered disulphide crosslinking and functional assays, our data suggest an unexpected peristaltic transport mechanism that is distinct from those observed in other ABC transporters.
PDB ID: 4FI3Download
MMDB ID: 103064
PDB Deposition Date: 2012/6/7
Updated in MMDB: 2012/11
Experimental Method:
x-ray diffraction
Resolution: 3.47  Å
Source Organism:
Similar Structures:
Biological Unit for 4FI3: pentameric; determined by author and by software (PISA)
Molecular Components in 4FI3
Label Count Molecule
Proteins (5 molecules)
2
Vitamin B12 Import System Permease Protein Btuc(Gene symbol: btuC)
Molecule annotation
2
Vitamin B12 Import Atp-binding Protein Btud(Gene symbol: btuD)
Molecule annotation
1
Vitamin B12-binding Protein(Gene symbol: btuF)
Molecule annotation
Chemicals (4 molecules)
1
2
2
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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