4FD9: Crystal Structure Of The Third Beta-gamma-crystallin Domain Of Crybg3 (betagamma-crystallin Domain-containing Protein 3) From Mus Musculus

Citation:
Abstract
The folding and unfolding of structurally similar proteins belonging to a family have long been a focus of investigation of the structure-(un)folding relationship. Such studies are yet to reach a consensus about whether structurally similar domains follow common or different unfolding pathways. Members of the betagamma-crystallin superfamily, which consists of structurally similar proteins with limited sequence similarity from diverse life forms spanning microbes to mammals, form an appropriate model system for exploring this relationship further. We selected a new member, Crybg3_D3, the third betagamma-crystallin domain of non-lens vertebrate protein Crybg3 from mouse brain. The crystal structure determined at 1.86 A demonstrates that the betagamma-crystallin domain of Crybg3 resembles more closely the lens betagamma-crystallins than the microbial crystallins do. However, interestingly, this structural cousin follows a quite distinct (un)folding pathway via formation of an intermediate state. The intermediate species is in a nativelike conformation with variation in flexibility and tends to form insoluble aggregates. The individual domains of lens betagamma-crystallins (and microbial homologues) do not follow such an unfolding pattern. Thus, even the closest members of a subfamily within a superfamily do not necessarily follow similar unfolding paths, suggesting the divergence acquired by these domains, which could be observed only by unfolding. Additionally, this study provides insights into the modifications that this domain has undergone during its recruitment into the non-lens tissues in vertebrates.
PDB ID: 4FD9Download
MMDB ID: 109005
PDB Deposition Date: 2012/5/26
Updated in MMDB: 2013/04
Experimental Method:
x-ray diffraction
Resolution: 1.86  Å
Source Organism:
Similar Structures:
Biological Unit for 4FD9: monomeric; determined by author
Molecular Components in 4FD9
Label Count Molecule
Protein (1 molecule)
1
Beta/gamma Crystallin Domain-containing Protein 3(Gene symbol: Crybg3)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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