4EQ2: Crystal Structure Analysis Of Chicken Interferon Gamma Receptor Alpha Chain

Citation:
Abstract
Interferon gamma (IFN-gamma) is an important cytokine that induces antiviral, antiproliferative, and immunomodulatory effects on target cells, and is also crucial in the early defense against intracellular parasites, such as Listeria monocytogenes and Toxoplasma gondii. The biological activity of IFN-gamma relies upon the formation of a complex with its 2 receptors, the interferon gamma alpha chain (IFNGR1) and beta chain (IFNGR2), which are type II cytokine receptors. Structural models of ligand-receptor interaction and complex structure of chicken IFNs with their receptors have remained elusive. Here we report the first structure of Gallus gallus (chicken) IFNGR1 (chIFNGR1) at 2.0 A by molecule replacement according to the structure of selenomethionine substituted chIFNGR1. The structural comparison reveals its structural similarities with other class II cytokine receptors, despite divergent primary sequences. We further investigate the ligand-receptor interaction properties of chicken IFN-gamma (chIFN-gamma) and chIFNGR1 using size-exclusion chromatography and surface plasmon resonance techniques. These data aid in the understanding of the interaction of chicken (avian) IFN-gamma with its receptors and its signal transduction.
PDB ID: 4EQ2Download
MMDB ID: 109371
PDB Deposition Date: 2012/4/18
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Similar Structures:
Biological Unit for 4EQ2: monomeric; determined by author and by software (PISA)
Molecular Components in 4EQ2
Label Count Molecule
Protein (1 molecule)
1
Interferon Gamma Receptor 1
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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