4EN6: Crystal Structure Of Ha70 (ha3) Subcomponent Of Clostridium Botulinum Type C Progenitor Toxin In Complex With Alpha 2-3-sialyllactose

Citation:
Abstract
Clostridium botulinum produces the botulinum neurotoxin, forming a large complex as progenitor toxins in association with non-toxic non-hemagglutinin and/or several different hemagglutinin (HA) subcomponents, HA33, HA17 and HA70, which bind to carbohydrate of glycoproteins from epithelial cells in the infection process. To elucidate the carbohydrate recognition mechanism of HA70, X-ray structures of HA70 from type C toxin (HA70/C) in complexes with sialylated oligosaccharides were determined, and a binding assay by the glycoconjugate microarray was performed. These results suggested that HA70/C can recognize both alpha2-3- and alpha2-6-sialylated oligosaccharides, and that it has a higher affinity for alpha2-3-sialylated oligosaccharides.
PDB ID: 4EN6Download
MMDB ID: 100383
PDB Deposition Date: 2012/4/12
Updated in MMDB: 2013/07
Experimental Method:
x-ray diffraction
Resolution: 2.56  Å
Source Organism:
Similar Structures:
Biological Unit for 4EN6: hexameric; determined by author and by software (PISA)
Molecular Components in 4EN6
Label Count Molecule
Proteins (6 molecules)
3
Hemagglutinin Components Ha-22/23/53
Molecule annotation
3
Hemagglutinin Components Ha-22/23/53
Molecule annotation
Chemicals (21 molecules)
1
15
2
3
3
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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