4DM7: Crystal structure of the CFTR inhibitory factor Cif with the E153D mutation

Citation:
Abstract
Opportunistic pathogens exploit diverse strategies to sabotage host defenses. Pseudomonas aeruginosa secretes the CFTR inhibitory factor Cif and thus triggers loss of CFTR, an ion channel required for airway mucociliary defense. However, the mechanism of action of Cif has remained unclear. It catalyzes epoxide hydrolysis, but there is no known role for natural epoxides in CFTR regulation. It was demonstrated that the hydrolase activity of Cif is strictly required for its effects on CFTR. A small-molecule inhibitor that protects this key component of the mucociliary defense system was also uncovered. These results provide a basis for targeting the distinctive virulence chemistry of Cif and suggest an unanticipated role of physiological epoxides in intracellular protein trafficking.
PDB ID: 4DM7Download
MMDB ID: 112397
PDB Deposition Date: 2012/2/7
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.36  Å
Source Organism:
Similar Structures:
Biological Unit for 4DM7: dimeric; determined by author and by software (PISA)
Molecular Components in 4DM7
Label Count Molecule
Proteins (2 molecules)
2
Putative Hydrolase
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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