National Center for
4DHM: Small-molecule Inhibitors Of 14-3-3 Protein-protein Interactions From Virtual Screening
Virtual screening and experimental validation reveal novel small-molecule inhibitors of 14-3-3 protein-protein interactions
Chem. Commun. (Camb. ) (2013) 49 p.8468-8470
We report first non-covalent and exclusively extracellular inhibitors of 14-3-3 protein-protein interactions identified by virtual screening. Optimization by crystal structure analysis and in vitro binding assays yielded compounds capable of disrupting the interaction of 14-3-3sigma with aminopeptidase N in a cellular assay.