4D0L: Phosphatidylinositol 4-kinase III beta-PIK93 in a complex with Rab11a- GTP gammaS

Phosphatidylinositol 4-kinases (PI4Ks) and small guanosine triphosphatases (GTPases) are essential for processes that require expansion and remodeling of phosphatidylinositol 4-phosphate (PI4P)-containing membranes, including cytokinesis, intracellular development of malarial pathogens, and replication of a wide range of RNA viruses. However, the structural basis for coordination of PI4K, GTPases, and their effectors is unknown. Here, we describe structures of PI4Kbeta (PI4KIIIbeta) bound to the small GTPase Rab11a without and with the Rab11 effector protein FIP3. The Rab11-PI4KIIIbeta interface is distinct compared with known structures of Rab complexes and does not involve switch regions used by GTPase effectors. Our data provide a mechanism for how PI4KIIIbeta coordinates Rab11 and its effectors on PI4P-enriched membranes and also provide strategies for the design of specific inhibitors that could potentially target plasmodial PI4KIIIbeta to combat malaria.
PDB ID: 4D0LDownload
MMDB ID: 120260
PDB Deposition Date: 2014/4/29
Updated in MMDB: 2018/06
Experimental Method:
x-ray diffraction
Resolution: 2.94  Å
Source Organism:
Similar Structures:
Biological Unit for 4D0L: dimeric; determined by software (PISA)
Molecular Components in 4D0L
Label Count Molecule
Proteins (3 molecules)
Phosphatidylinositol 4-kinase Beta(Gene symbol: PI4KB)
Molecule annotation
Ras-related Protein Rab-11a(Gene symbol: RAB11A)
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB