4CVU: Structure Of Fungal Beta-mannosidase From Glycoside Hydrolase Family 2 Of Trichoderma Harzianum

Citation:
Abstract
Hemicellulose is an important part of the plant cell wall biomass, and is relevant to cellulosic ethanol technologies. beta-Mannosidases are enzymes capable of cleaving nonreducing residues of beta-d-mannose from beta-d-mannosides and hemicellulose mannose-containing polysaccharides, such as mannans and galactomannans. beta-Mannosidases are distributed between glycoside hydrolase (GH) families 1, 2, and 5, and only a handful of the enzymes have been structurally characterized to date. The only published X-ray structure of a GH family 2 mannosidase is that of the bacterial Bacteroides thetaiotaomicron enzyme. No structures of eukaryotic mannosidases of this family are currently available. To fill this gap, we set out to solve the structure of Trichoderma harzianum GH family 2 beta-mannosidase and to refine it to 1.9-A resolution. Structural comparisons of the T. harzianum GH2 beta-mannosidase highlight similarities in its structural architecture with other members of GH family 2, reveal the molecular mechanism of beta-mannoside binding and recognition, and shed light on its putative galactomannan-binding site. DATABASE: Coordinates and observed structure factor amplitudes have been deposited with the Protein Data Bank (4CVU and 4UOJ). The T. harzianum beta-mannosidase 2A nucleotide sequence has GenBank accession number BankIt1712036 GeneMark.hmm KJ624918.
PDB ID: 4CVUDownload
MMDB ID: 121299
PDB Deposition Date: 2014/3/31
Updated in MMDB: 2014/08
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 4CVU: dimeric; determined by software (PISA)
Molecular Components in 4CVU
Label Count Molecule
Proteins (2 molecules)
2
Beta-mannosidase
Molecule annotation
Chemicals (114 molecules)
1
30
2
8
3
24
4
34
5
10
6
2
7
2
8
2
9
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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