4CSF: Structural Insights Into Toscana Virus Rna Encapsidation

Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses.
PDB ID: 4CSFDownload
MMDB ID: 118977
PDB Deposition Date: 2014/3/7
Updated in MMDB: 2014/06
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4CSF: nonameric; determined by author and by software (PISA)
Molecular Components in 4CSF
Label Count Molecule
Proteins (6 molecules)
Nucleoprotein(Gene symbol: SFNVsSgp2)
Molecule annotation
Nucleoprotein(Gene symbol: SFNVsSgp2)
Molecule annotation
Nucleotide(1 molecule)
RNA (5'-r(*up*gp*up*gp*up*up*up*cp*up)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB