4CRV: Complex Of Human Cnot9 And Cnot1 Including Two Tryptophans

Citation:
Abstract
CCR4-NOT is a major effector complex in miRNA-mediated gene silencing. It is recruited to miRNA targets through interactions with tryptophan (W)-containing motifs in TNRC6/GW182 proteins and is required for both translational repression and degradation of miRNA targets. Here, we elucidate the structural basis for the repressive activity of CCR4-NOT and its interaction with TNRC6/GW182s. We show that the conserved CNOT9 subunit attaches to a domain of unknown function (DUF3819) in the CNOT1 scaffold. The resulting complex provides binding sites for TNRC6/GW182, and its crystal structure reveals tandem W-binding pockets located in CNOT9. We further show that the CNOT1 MIF4G domain interacts with the C-terminal RecA domain of DDX6, a translational repressor and decapping activator. The crystal structure of this complex demonstrates striking similarity to the eIF4G-eIF4A complex. Together, our data provide the missing physical links in a molecular pathway that connects miRNA target recognition with translational repression, deadenylation, and decapping.
PDB ID: 4CRVDownload
MMDB ID: 119527
PDB Deposition Date: 2014/3/1
Updated in MMDB: 2014/06
Experimental Method:
x-ray diffraction
Resolution: 2.05  Å
Source Organism:
Similar Structures:
Biological Unit for 4CRV: dimeric; determined by author and by software (PISA)
Molecular Components in 4CRV
Label Count Molecule
Proteins (2 molecules)
1
Ccr4-not Transcription Complex Subunit 1(Gene symbol: CNOT1)
Molecule annotation
1
Cell Differentiation Protein Rcd1 Homolog(Gene symbol: CNOT9)
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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