4C2W: Crystal Structure Of Aurora B In Complex With Amp-pnp

Citation:
Abstract
The Aurora family is a well conserved and well characterized group of serine-threonine kinases involved in the normal progression of mitosis. The deregulation of Aurora kinases impairs spindle assembly, checkpoint function and cell division. To date, many small molecules that compete with ATP for binding to Aurora kinases have been developed and characterized. Here, the first structure of the Xenopus laevis Aurora B-INCENP complex bound to the clinically relevant small molecule barasertib was determined. The binding properties of this inhibitor to the Aurora B active site are analyzed and reported. An unexpected crystal-packing contact in the Aurora B-INCENP structure coordinated by an ATP analogue is also reported, in which the INCENP C-terminus occupies the substrate-binding region, resembling the protein kinase A inhibitory mechanism.
PDB ID: 4C2WDownload
MMDB ID: 117782
PDB Deposition Date: 2013/8/20
Updated in MMDB: 2014/03
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Similar Structures:
Biological Unit for 4C2W: dimeric; determined by author and by software (PISA)
Molecular Components in 4C2W
Label Count Molecule
Proteins (2 molecules)
1
Aurora Kinase B-A(Gene symbol: aurkb.L)
Molecule annotation
1
Inner Centromere Protein a(Gene symbol: incenp.L)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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