4B6A: Cryo-em Structure of the 60S Ribosomal Subunit in Complex With Arx1 and Rei1

Citation:
Abstract
Eukaryotic ribosome biogenesis requires many protein factors that facilitate the assembly, nuclear export and final maturation of 40S and 60S particles. We have biochemically characterized ribosomal complexes of the yeast 60S-biogenesis factor Arx1 and late-maturation factors Rei1 and Jjj1 and determined their cryo-EM structures. Arx1 was visualized bound to the 60S subunit together with Rei1, at 8.1-A resolution, to reveal the molecular details of Arx1 binding whereby Arx1 arrests the eukaryotic-specific rRNA expansion segment 27 near the polypeptide tunnel exit. Rei1 and Jjj1, which have been implicated in Arx1 recycling, bind in the vicinity of Arx1 and form a network of interactions. We suggest that, in addition to the role of Arx1 during pre-60S nuclear export, the binding of Arx1 conformationally locks the pre-60S subunit and inhibits the premature association of nascent chain-processing factors to the polypeptide tunnel exit.
MMDB ID: 105292
PDB Deposition Date: 2012/8/7
Updated in MMDB: 2012/12 
Experimental Method:
electron microscopy
Resolution: 8.1  Å
Source Organism:
Saccharomyces cerevisiae S288C
Similar Structures:
Merged PDB IDs:4B6A 4B6B
Biological Unit for 4B6A: 50-meric
Molecular Components in 4B6A
Label Count Molecule
Proteins (46 molecules)
1
60S Ribosomal Protein L2-b
Molecule annotation
1
60S Ribosomal Protein L3
Molecule annotation
1
60S Ribosomal Protein L4-a
Molecule annotation
1
60S Ribosomal Protein L5
Molecule annotation
1
60S Ribosomal Protein L6-a
Molecule annotation
1
60S Ribosomal Protein L7-a
Molecule annotation
1
60S Ribosomal Protein L8-a
Molecule annotation
1
60S Ribosomal Protein L9-a
Molecule annotation
1
60S Ribosomal Protein L10
Molecule annotation
1
60S Ribosomal Protein L11-b
Molecule annotation
1
60S Ribosomal Protein L12
Molecule annotation
1
60S Ribosomal Protein L13-a
Molecule annotation
1
60S Ribosomal Protein L14-a
Molecule annotation
1
60S Ribosomal Protein L15-a
Molecule annotation
1
60S Ribosomal Protein L16-a
Molecule annotation
1
60S Ribosomal Protein L17-a
Molecule annotation
1
60S Ribosomal Protein L18-b
Molecule annotation
1
60S Ribosomal Protein L19-b
Molecule annotation
1
60S Ribosomal Protein L20-a
Molecule annotation
1
60S Ribosomal Protein L21-a
Molecule annotation
1
60S Ribosomal Protein L22-a
Molecule annotation
1
60S Ribosomal Protein L23-a
Molecule annotation
1
60S Ribosomal Protein L24-a
Molecule annotation
1
60S Ribosomal Protein L25
Molecule annotation
1
60S Ribosomal Protein L26-a
Molecule annotation
1
60S Ribosomal Protein L27-a
Molecule annotation
1
60S Ribosomal Protein L28
Molecule annotation
1
60S Ribosomal Protein L29
Molecule annotation
1
60S Ribosomal Protein L32
Molecule annotation
1
60S Ribosomal Protein L31-a
Molecule annotation
1
60S Ribosomal Protein L30
Molecule annotation
1
60S Ribosomal Protein L33-a
Molecule annotation
1
60S Ribosomal Protein L34-a
Molecule annotation
1
60S Ribosomal Protein L35-a
Molecule annotation
1
60S Ribosomal Protein L36-a
Molecule annotation
1
60S Ribosomal Protein L37-a
Molecule annotation
1
60S Ribosomal Protein L38
Molecule annotation
1
60S Ribosomal Protein L39
Molecule annotation
1
60S Ribosomal Protein L40
Molecule annotation
1
60S Ribosomal Protein L41-a
Molecule annotation
1
60S Ribosomal Protein L42-a
Molecule annotation
1
60S Ribosomal Protein L43-a
Molecule annotation
1
60S Acidic Ribosomal Protein P0
Molecule annotation
1
Ribosomal Protein P1 Alpha
Molecule annotation
1
Ribosomal Protein P2 Beta
Molecule annotation
1
Probable Metalloprotease Arx1
Molecule annotation
Nucleotides(4 molecules)
1
Es27 of the 25S rRNA
Molecule annotation
1
25S Ribosomal RNA
Molecule annotation
1
5S Ribosomal RNA
Molecule annotation
1
5.8s Ribosomal RNA
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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