4B3Q: Structures Of Hiv-1 Rt And Rna-dna Complex Reveal A Unique Rt Conformation And Substrate Interface

Citation:
Abstract
Hundreds of structures of type 1 human immunodeficiency virus (HIV-1) reverse transcriptase (RT) have been determined, but only one contains an RNA/DNA hybrid. Here we report three structures of HIV-1 RT complexed with a non-nucleotide RT inhibitor (NNRTI) and an RNA/DNA hybrid. In the presence of an NNRTI, the RNA/DNA structure differs from all prior nucleic acid-RT structures including the RNA/DNA hybrid. The enzyme structure also differs from all previous RT-DNA complexes. Thus, the hybrid has ready access to the RNase-H active site. These observations indicate that an RT-nucleic acid complex may adopt two structural states, one competent for DNA polymerization and the other for RNA degradation. RT mutations that confer drug resistance but are distant from the inhibitor-binding sites often map to the unique RT-hybrid interface that undergoes conformational changes between two catalytic states.
PDB ID: 4B3QDownload
MMDB ID: 106666
PDB Deposition Date: 2012/7/25
Updated in MMDB: 2013/02
Experimental Method:
x-ray diffraction
Resolution: 5  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 4B3Q: tetrameric; determined by author and by software (PISA)
Molecular Components in 4B3Q
Label Count Molecule
Proteins (2 molecules)
1
Reverse Transcriptase/ribonuclease H
Molecule annotation
1
P51 RT
Molecule annotation
Nucleotides(2 molecules)
1
Primer DNA
Molecule annotation
1
Template RNA
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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