4B3N: Crystal Structure Of Rhesus Trim5alpha Pry/spry Domain

Tripartite motif protein isoform 5 alpha (TRIM5alpha) is a potent antiviral protein that restricts infection by HIV-1 and other retroviruses. TRIM5alpha recognizes the lattice of the retrovirus capsid through its B30.2 (PRY/SPRY) domain in a species-specific manner. Upon binding, TRIM5alpha induces premature disassembly of the viral capsid and activates the downstream innate immune response. We have determined the crystal structure of the rhesus TRIM5alpha PRY/SPRY domain that reveals essential features for capsid binding. Combined cryo-electron microscopy and biochemical data show that the monomeric rhesus TRIM5alpha PRY/SPRY, but not the human TRIM5alpha PRY/SPRY, can bind to HIV-1 capsid protein assemblies without causing disruption of the capsid. This suggests that the PRY/SPRY domain alone constitutes an important pattern-sensing component of TRIM5alpha that is capable of interacting with viral capsids of different curvatures. Our results provide molecular insights into the mechanisms of TRIM5alpha-mediated retroviral restriction.
PDB ID: 4B3NDownload
MMDB ID: 104230
PDB Deposition Date: 2012/7/25
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 3.3  Å
Similar Structures:
Biological Unit for 4B3N: monomeric; determined by author and by software (PISA)
Molecular Components in 4B3N
Label Count Molecule
Protein (1 molecule)
Maltose-binding Periplasmic Protein, Tripartite Motif- Containing Protein 5
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB