4AOB: Sam-I Riboswitch Containing The T. Solenopsae Kt-23 In Complex With S-Adenosyl Methionine

Citation:
Abstract
The kink turn (k-turn) is a frequently occurring motif, comprising a bulge followed by G*A and A*G pairs that introduces a sharp axial bend in duplex RNA. Natural k-turn sequences exhibit significant departures from the consensus, including the A*G pairs that form critical interactions stabilizing the core of the structure. Kt-23 found in the small ribosomal subunit differs from the consensus in many organisms, particularly in the second A*G pair distal to the bulge (2b*2n). Analysis of many Kt-23 sequences shows that the frequency of occurrence at the 2n position (i.e., on the nonbulged strand, normally G in standard k-turns) is U>C>G>A. Less than 1% of sequences have A at the 2n position, but one such example occurs in Thelohania solenopsae Kt-23. This sequence folds only weakly in the presence of Mg(2+) ions but is induced to fold normally by the binding of L7Ae protein. Introduction of this sequence into the SAM-I riboswitch resulted in normal binding of SAM ligand, indicating that tertiary RNA contacts have resulted in k-turn folding. X-ray crystallography shows that the T. solenopsae Kt-23 adopts a standard k-turn geometry, making the key, conserved hydrogen bonds in the core and orienting the 1n (of the bulge-proximal A*G pair) and 2b adenine nucleobases in position facing the opposing minor groove. The 2b and 2n adenine nucleobases are not directly hydrogen bonded, but each makes hydrogen bonds to their opposing strands.
PDB ID: 4AOBDownload
MMDB ID: 99408
PDB Deposition Date: 2012/3/25
Updated in MMDB: 2012/05
Experimental Method:
x-ray diffraction
Resolution: 2.95  Å
Source Organism:
Biological Unit for 4AOB: dimeric; determined by software (PISA)
Molecular Components in 4AOB
Label Count Molecule
Nucleotide(1 molecule)
2
Sam-i Riboswitch
Molecule annotation
Chemicals (50 molecules)
1
28
2
14
3
6
4
2
* Click molecule labels to explore molecular sequence information.

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