4AB1: Recombinant Human Carboxylesterase 1 From Whole Cabbage Loopers

The use of whole insect larvae as a source of recombinant proteins offers a more cost-effective method of producing large quantities of human proteins than conventional cell-culture approaches. Human carboxylesterase 1 has been produced in and isolated from whole Trichoplusia ni larvae. The recombinant protein was crystallized and its structure was solved to 2.2 resolution. The results indicate that the larvae-produced enzyme is essentially identical to that isolated from cultured Sf21 cells, supporting the use of this expression system to produce recombinant enzymes for crystallization studies.
PDB ID: 4AB1Download
MMDB ID: 97827
PDB Deposition Date: 2011/12/6
Updated in MMDB: 2012/05
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 4AB1: hexameric; determined by author and by software (PISA)
Molecular Components in 4AB1
Label Count Molecule
Proteins (6 molecules)
Liver Carboxylesterase 1(Gene symbol: CES1)
Molecule annotation
Chemicals (24 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB