National Center for
4AB1: Recombinant Human Carboxylesterase 1 From Whole Cabbage Loopers
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2012) 68 p.269-272
The use of whole insect larvae as a source of recombinant proteins offers a more cost-effective method of producing large quantities of human proteins than conventional cell-culture approaches. Human carboxylesterase 1 has been produced in and isolated from whole Trichoplusia ni larvae. The recombinant protein was crystallized and its structure was solved to 2.2 resolution. The results indicate that the larvae-produced enzyme is essentially identical to that isolated from cultured Sf21 cells, supporting the use of this expression system to produce recombinant enzymes for crystallization studies.