4A35: Crystal Structure Of Human Mitochondrial Enolase Superfamily Member 1 (enosf1)

In humans, the gene encoding a reverse thymidylate synthase (rTS) is transcribed in the reverse direction of the gene encoding thymidylate synthase (TS) that is involved in DNA biosynthesis. Three isoforms are found: alpha, beta, and gamma, with the transcript of the alpha-isoform overlapping with that of TS. rTSbeta has been of interest since the discovery of its overexpression in methotrexate and 5-fluorouracil resistant cell lines. Despite more than 20 years of study, none of the rTS isoforms have been biochemically or structurally characterized. In this study, we identified rTSgamma as an l-fuconate dehydratase and determined its high-resolution crystal structure. Our data provide an explanation for the observed difference in enzymatic activities between rTSbeta and rTSgamma, enabling more informed proposals for the possible function of rTSbeta in chemotherapeutic resistance.
PDB ID: 4A35Download
MMDB ID: 94372
PDB Deposition Date: 2011/9/30
Updated in MMDB: 2015/12
Experimental Method:
x-ray diffraction
Resolution: 1.74  Å
Source Organism:
Similar Structures:
Biological Unit for 4A35: dimeric; determined by author and by software (PISA)
Molecular Components in 4A35
Label Count Molecule
Proteins (2 molecules)
Mitochondrial Enolase Superfamily Member 1(Gene symbol: ENOSF1)
Molecule annotation
Chemicals (18 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB