4A17: T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6

Citation:
Abstract
Protein synthesis in all organisms is catalyzed by ribosomes. In comparison to their prokaryotic counterparts, eukaryotic ribosomes are considerably larger and are subject to more complex regulation. The large ribosomal subunit (60S) catalyzes peptide bond formation and contains the nascent polypeptide exit tunnel. We present the structure of the 60S ribosomal subunit from Tetrahymena thermophila in complex with eukaryotic initiation factor 6 (eIF6), cocrystallized with the antibiotic cycloheximide (a eukaryotic-specific inhibitor of protein synthesis), at a resolution of 3.5 angstroms. The structure illustrates the complex functional architecture of the eukaryotic 60S subunit, which comprises an intricate network of interactions between eukaryotic-specific ribosomal protein features and RNA expansion segments. It reveals the roles of eukaryotic ribosomal protein elements in the stabilization of the active site and the extent of eukaryotic-specific differences in other functional regions of the subunit. Furthermore, it elucidates the molecular basis of the interaction with eIF6 and provides a structural framework for further studies of ribosome-associated diseases and the role of the 60S subunit in the initiation of protein synthesis.
MMDB ID: 99458
PDB Deposition Date: 2011/9/14
Updated in MMDB: 2012/08
Experimental Method:
x-ray diffraction
Resolution: 3.52  Å
Source Organism:
Similar Structures:
Merged PDB IDs:4A17 4A18 4A19 4A1A 4A1B 4A1C 4A1D 4A1E
Asymmetric Unit for 4A17: 184-meric
Molecular Components in 4A17
Label Count Molecule
Proteins (172 molecules)
4
Rpl8
Molecule annotation
4
Ribosomal Protein L3
Molecule annotation
4
Rpl4
Molecule annotation
4
60S Ribosomal Protein L11
Molecule annotation
4
60S Ribosomal Protein L9
Molecule annotation
4
Rpl7a
Molecule annotation
4
Rplp0
Molecule annotation
4
60S Ribosomal Protein L10
Molecule annotation
4
60S Ribosomal Protein L13a
Molecule annotation
4
Rpl23
Molecule annotation
4
60S Ribosomal Protein L27a
Molecule annotation
4
Ribosomal Protein L15
Molecule annotation
4
60S Ribosomal Protein L5
Molecule annotation
4
Rpl18
Molecule annotation
4
Rpl19
Molecule annotation
4
60S Ribosomal Protein L21
Molecule annotation
4
Rpl17
Molecule annotation
4
Rpl23a
Molecule annotation
4
Rpl26
Molecule annotation
4
Rpl24
Molecule annotation
4
Rpl35
Molecule annotation
4
60S Ribosomal Protein L7
Molecule annotation
4
60S Ribosomal Protein L31
Molecule annotation
4
60S Ribosomal Protein L32
Molecule annotation
4
Rpl37a
Molecule annotation
4
Ribosomal Protein L37
Molecule annotation
4
Rpl39
Molecule annotation
4
60S Ribosomal Protein L36a
Molecule annotation
4
Rpl6
Molecule annotation
4
Rpl14
Molecule annotation
4
Rpl30
Molecule annotation
4
Rpl35a
Molecule annotation
4
Translation Initiation Factor Eif-6, Putative Family Protein
Molecule annotation
4
Ubiquitin-60s Ribosomal Protein L40
Molecule annotation
4
Rpl34
Molecule annotation
4
Ribosomal Protein L22
Molecule annotation
4
Rpl27
Molecule annotation
4
Rpl28
Molecule annotation
4
Rpl38
Molecule annotation
4
60S Ribosomal Protein L36
Molecule annotation
4
Rpl29
Molecule annotation
4
Rpl13
Molecule annotation
4
Rpl18a
Molecule annotation
Nucleotides(3 molecules)
4
5.8s rRNA
Molecule annotation
4
5S rRNA
Molecule annotation
4
26S rRNA
Molecule annotation
Chemicals (885 molecules)
1
865
2
20
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.