3ZYN: Crystal Structure Of The N-Terminal Leucine Rich Repeats Of Netrin-G Ligand-3

Citation:
Abstract
Brain wiring depends on cells making highly localized and selective connections through surface protein-protein interactions, including those between NetrinGs and NetrinG ligands (NGLs). The NetrinGs are members of the structurally uncharacterized netrin family. We present a comprehensive crystallographic analysis comprising NetrinG1-NGL1 and NetrinG2-NGL2 complexes, unliganded NetrinG2 and NGL3. Cognate NetrinG-NGL interactions depend on three specificity-conferring NetrinG loops, clasped tightly by matching NGL surfaces. We engineered these NGL surfaces to implant custom-made affinities for NetrinG1 and NetrinG2. In a cellular patterning assay, we demonstrate that NetrinG-binding selectivity can direct the sorting of a mixed population of NGLs into discrete cell surface subdomains. These results provide a molecular model for selectivity-based patterning in a neuronal recognition system, dysregulation of which is associated with severe neuropsychological disorders.
PDB ID: 3ZYNDownload
MMDB ID: 94129
PDB Deposition Date: 2011/8/23
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 3.2  Å
Source Organism:
Similar Structures:
Biological Unit for 3ZYN: monomeric; determined by author and by software (PISA)
Molecular Components in 3ZYN
Label Count Molecule
Protein (1 molecule)
1
Leucine-rich Repeat-containing Protein 4B(Gene symbol: Lrrc4b)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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