3ZYL: Structure Of A Truncated Calm (Picalm) Anth Domain

Citation:
Abstract
SNAREs provide a large part of the specificity and energy needed for membrane fusion and, to do so, must be localized to their correct membranes. Here, we show that the R-SNAREs VAMP8, VAMP3, and VAMP2, which cycle between the plasma membrane and endosomes, bind directly to the ubiquitously expressed, PtdIns4,5P(2)-binding, endocytic clathrin adaptor CALM/PICALM. X-ray crystallography shows that the N-terminal halves of their SNARE motifs bind the CALM(ANTH) domain as helices in a manner that mimics SNARE complex formation. Mutation of residues in the CALM:SNARE interface inhibits binding in vitro and prevents R-SNARE endocytosis in vivo. Thus, CALM:R-SNARE interactions ensure that R-SNAREs, required for the fusion of endocytic clathrin-coated vesicles with endosomes and also for subsequent postendosomal trafficking, are sorted into endocytic vesicles. CALM's role in directing the endocytosis of small R-SNAREs may provide insight into the association of CALM/PICALM mutations with growth retardation, cognitive defects, and Alzheimer's disease.
PDB ID: 3ZYLDownload
MMDB ID: 95622
PDB Deposition Date: 2011/8/23
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Similar Structures:
Biological Unit for 3ZYL: dimeric; determined by author and by software (PISA)
Molecular Components in 3ZYL
Label Count Molecule
Proteins (2 molecules)
2
Phosphatidylinositol-binding Clathrin Assembly Protein(Gene symbol: Picalm)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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