3ZR1: Crystal Structure Of Human Mth1

Citation:
Abstract
MTH1 hydrolyzes oxidized nucleotide triphosphates, thereby preventing them from being incorporated into DNA. We here present the structures of human MTH1 (1.9A) and its complex with the product 8-oxo-dGMP (1.8A). Unexpectedly MTH1 binds the nucleotide in the anti conformation with no direct interaction between the 8-oxo group and the protein. We suggest that the specificity depends on the stabilization of an enol tautomer of the 8-oxo form of dGTP. The binding of the product induces no major structural changes. The structures reveal the mode of nucleotide binding in MTH1 and provide the structural basis for inhibitor design.
PDB ID: 3ZR1Download
MMDB ID: 93011
PDB Deposition Date: 2011/6/13
Updated in MMDB: 2011/08
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 3ZR1: monomeric; determined by author and by software (PISA)
Molecular Components in 3ZR1
Label Count Molecule
Protein (1 molecule)
1
7,8-dihydro-8-oxoguanine Triphosphatase(Gene symbol: NUDT1)
Molecule annotation
Chemicals (4 molecules)
1
1
2
3
* Click molecule labels to explore molecular sequence information.

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