3ZQW: Structure of CBM3b of major scaffoldin subunit ScaA from Acetivibrio cellulolyticus

Citation:
Abstract
The carbohydrate-binding module (CBM) of the major scaffoldin subunit ScaA of the cellulosome of Acetivibrio cellulolyticus is classified as a family 3b CBM and binds strongly to cellulose. The CBM3b was overexpressed, purified and crystallized, and its three-dimensional structure was determined. The structure contained a nickel-binding site located at the N-terminal region in addition to a 'classical' CBM3b calcium-binding site. The structure was also determined independently by the SAD method using data collected at the Ni-absorption wavelength of 1.48395 A and even at a wavelength of 0.97625 A in a favourable case. The new scaffoldin-borne CBM3 structure reported here provides clear evidence for the proposition that a family 3b CBM may be accommodated in scaffoldin subunits and functions as the major substrate-binding entity of the cellulosome assembly.
PDB ID: 3ZQWDownload
MMDB ID: 96414
PDB Deposition Date: 2011/6/12
Updated in MMDB: 2018/07
Experimental Method:
x-ray diffraction
Resolution: 1.07  Å
Source Organism:
Similar Structures:
Biological Unit for 3ZQW: monomeric; determined by author and by software (PISA)
Molecular Components in 3ZQW
Label Count Molecule
Protein (1 molecule)
1
Cellulosomal Scaffoldin
Molecule annotation
Chemicals (4 molecules)
1
1
2
1
3
1
4
1
* Click molecule labels to explore molecular sequence information.

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