3ZO5: Structure Of Senp2-loop1 In Complex With Presumo-2

Citation:
Abstract
The SENP proteases regulate the SUMO conjugates in the cell by cleaving SUMO from target proteins. SENP6 and SENP7 are the most divergent members of the SENP/ULP protease family in humans by the presence of insertions in their catalytic domains. Loop1 insertion is determinant for the SUMO2/3 activity and specificity on SENP6 and SENP7. To gain structural insights into the role of Loop1, we have designed a chimeric SENP2 with the insertion of Loop1 into its sequence. The structure of SENP2-Loop1 in complex with SUMO2 was solved at 2.15 A resolution, and reveals the details of an interface exclusive to SENP6/7 and the formation of unique contacts between both proteins. Interestingly, functional data with SUMO substrates showed an increase of the proteolytic activity in the SENP2-Loop1 chimera for diSUMO2 and polySUMO2 substrates.
PDB ID: 3ZO5Download
MMDB ID: 116962
PDB Deposition Date: 2013/2/20
Updated in MMDB: 2014/04
Experimental Method:
x-ray diffraction
Resolution: 2.15  Å
Source Organism:
Similar Structures:
Biological Unit for 3ZO5: dimeric; determined by author and by software (PISA)
Molecular Components in 3ZO5
Label Count Molecule
Proteins (2 molecules)
1
Sentrin-specific Protease 2(Gene symbol: SENP2)
Molecule annotation
1
Small Ubiquitin-related Modifier 2(Gene symbol: SUMO2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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