3WTY: Crystal structure of the complex comprised of ETS1(G333P), RUNX1, CBFBETA, and the tcralpha gene enhancer DNA

Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate specification, and differentiation through precise and complicated transcriptional mechanisms. Chemical modifications, such as phosphorylation, of TFs induced by cell signaling further modulate the dynamic cooperativity of TFs. In this study, we found that various Ets1-containing TF-DNA complexes respond differently to calcium-induced phosphorylation of Ets1, which is known to inhibit Ets1-DNA binding. Crystallographic analysis of a complex comprising Ets1, Runx1, and CBFbeta at the TCRalpha enhancer revealed that Ets1 acquires robust binding stability in the Runx1 and DNA-complexed state, via allosteric mechanisms. This allows phosphorylated Ets1 to be retained at the TCRalpha enhancer with Runx1, in contrast to other Ets1 target gene enhancers including mb-1 and stromelysin-1. This study provides a structure-based model for cell-signaling-dependent regulation of target genes, mediated via chemical modification of TFs.
PDB ID: 3WTYDownload
MMDB ID: 122285
PDB Deposition Date: 2014/4/21
Updated in MMDB: 2017/12
Experimental Method:
x-ray diffraction
Resolution: 2.7  Å
Source Organism:
Mus musculus
Similar Structures:
Biological Unit for 3WTY: pentameric; determined by author and by software (PISA)
Molecular Components in 3WTY
Label Count Molecule
Proteins (3 molecules)
Runt-related Transcription Factor 1(Gene symbol: Runx1)
Molecule annotation
Core-binding Factor Subunit Beta(Gene symbol: Cbfb)
Molecule annotation
Protein C-ets-1(Gene symbol: ETS1)
Molecule annotation
Nucleotides(2 molecules)
DNA (5'-d(*gp*ap*ap*gp*cp*cp*ap*cp*ap*tp*cp*cp*tp*cp*t)-3')
Molecule annotation
DNA (5'-d(*ap*gp*ap*gp*gp*ap*tp*gp*tp*gp*gp*cp*tp*tp*c)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB