3WTP: Crystal Structure of the heterotypic nucleosome containing human CENP-A and H3.3

The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In the present study, we report the crystal structure of the heterotypic CENP-A/H3.3 particle and reveal its "hybrid structure", in which the physical characteristics of CENP-A and H3.3 are conserved independently within the same particle. The CENP-A/H3.3 nucleosome forms an unexpectedly stable structure as compared to the CENP-A nucleosome, and allows the binding of the essential centromeric protein, CENP-C, which is ectopically mislocalized in the chromosomes of CENP-A overexpressing cells.
PDB ID: 3WTPDownload
MMDB ID: 125172
PDB Deposition Date: 2014/4/14
Updated in MMDB: 2017/12
Experimental Method:
x-ray diffraction
Resolution: 2.67  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 3WTP: decameric; determined by author and by software (PISA)
Molecular Components in 3WTP
Label Count Molecule
Proteins (8 molecules)
Histone H3-like Centromeric Protein a(Gene symbol: CENPA)
Molecule annotation
Histone H4(Gene symbol: HIST1H4I)
Molecule annotation
Histone H2A Type 1-b/e(Gene symbol: HIST1H2AE)
Molecule annotation
Histone H2B Type 1-J(Gene symbol: HIST1H2BJ)
Molecule annotation
Histone H3.3(Gene symbol: H3F3A)
Molecule annotation
Nucleotide(1 molecule)
DNA (146-mer)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB