National Center for
3WLF: Crystal structure of (R)-carbonyl reductase from Candida Parapsilosis in complex with (R)-1-phenyl-1,2-ethanediol
Unconserved substrate-binding sites direct the stereoselectivity of medium-chain alcohol dehydrogenase
Chem. Commun. (Camb. ) (2014) 50 p.7770-7772
Structure-guided design of substrate-binding pocket inversed the stereoselectivity of an NADH-dependent medium-chain alcohol dehydrogenase (MDR) from Prelog to anti-Prelog. The pocket-forming amino acids, especially the unconserved residues as hotspots, play critical roles in directing MDRs' stereoselectivity.