3WGT: Crystal Structure Of D-amino Acid Oxidase Mutant

Citation:
Abstract
The deracemization of racemic amines to yield enantioenriched amines using S-stereoselective amine oxidases (AOx) has recently been attracting attention. However, R-stereoselective AOx that are suitable for deracemization have not yet been identified. An R-stereoselective AOx was now evolved from porcine kidney D-amino acid oxidase (pkDAO) and subsequently use for the deracemization of racemic amines. The engineered pkDAO, which was obtained by directed evolution, displayed a markedly changed substrate specificity towards R amines. The mutant enzyme exhibited a high preference towards the substrate alpha-methylbenzylamine and was used to synthesize the S amine through deracemization. The findings of this study indicate that further investigations on the structure-activity relationship of AOx are warranted and also provide a new method for biotransformations in organic synthesis.
PDB ID: 3WGTDownload
MMDB ID: 118716
PDB Deposition Date: 2013/8/9
Updated in MMDB: 2014/04
Experimental Method:
x-ray diffraction
Resolution: 1.88  Å
Source Organism:
Similar Structures:
Biological Unit for 3WGT: dimeric; determined by author and by software (PISA)
Molecular Components in 3WGT
Label Count Molecule
Proteins (2 molecules)
2
D-amino-acid Oxidase(Gene symbol: DAO)
Molecule annotation
Chemicals (8 molecules)
1
2
2
2
3
4
* Click molecule labels to explore molecular sequence information.

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