3WAF: X-ray Structure Of Apo-ttfbpa, A Ferric Ion-binding Protein From Thermus Thermophilus Hb8

Crystal structures of FbpA, the periplasmic ferric ion-binding protein of an iron-uptake ABC transporter, from Thermus thermophilus HB8 (TtFbpA) have been solved in apo and ferric ion-bound forms at 1.8 and 1.7 A resolution, respectively. The latter crystal structure shows that the bound ferric ion forms a novel six-coordinated complex with three tyrosine side chains, two bicarbonates and a water molecule in the metal-binding site. The results of gel-filtration chromatography and dynamic light scattering show that TtFbpA exists as a monomer in solution regardless of ferric ion binding and that TtFbpA adopts a more compact conformation in the ferric ion-bound state than in the apo state in solution.
PDB ID: 3WAFDownload
MMDB ID: 119997
PDB Deposition Date: 2013/5/2
Updated in MMDB: 2014/05
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 3WAF: dimeric; determined by author and by software (PISA)
Molecular Components in 3WAF
Label Count Molecule
Proteins (2 molecules)
Iron ABC Transporter, Periplasmic Iron-binding Protein
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB