3WAA: The Nucleosome Containing Human H2a.z.2

Citation:
Abstract
The histone H2A.Z variant is widely conserved among eukaryotes. Two isoforms, H2A.Z.1 and H2A.Z.2, have been identified in vertebrates and may have distinct functions in cell growth and gene expression. However, no structural differences between H2A.Z.1 and H2A.Z.2 have been reported. In the present study, the crystal structures of nucleosomes containing human H2A.Z.1 and H2A.Z.2 were determined. The structures of the L1 loop regions were found to clearly differ between H2A.Z.1 and H2A.Z.2, although their amino-acid sequences in this region are identical. This structural polymorphism may have been induced by a substitution that evolutionally occurred at the position of amino acid 38 and by the flexible nature of the L1 loops of H2A.Z.1 and H2A.Z.2. It was also found that in living cells nucleosomal H2A.Z.1 exchanges more rapidly than H2A.Z.2. A mutational analysis revealed that the amino-acid difference at position 38 is at least partially responsible for the distinctive dynamics of H2A.Z.1 and H2A.Z.2. These findings provide important new information for understanding the differences in the regulation and functions of H2A.Z.1 and H2A.Z.2 in cells.
PDB ID: 3WAADownload
MMDB ID: 115988
PDB Deposition Date: 2013/4/30
Updated in MMDB: 2013/12
Experimental Method:
x-ray diffraction
Resolution: 3.2  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 3WAA: decameric; determined by author and by software (PISA)
Molecular Components in 3WAA
Label Count Molecule
Proteins (8 molecules)
2
Histone H3.1(Gene symbol: HIST1H3A)
Molecule annotation
2
Histone H4(Gene symbol: HIST1H4I)
Molecule annotation
2
Histone H2a.v(Gene symbol: H2AFV)
Molecule annotation
2
Histone H2B Type 1-J(Gene symbol: HIST1H2BJ)
Molecule annotation
Nucleotide(1 molecule)
2
DNA (146-mer)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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