National Center for
3V82: Thaumatin By Lb Based Hanging Drop Vapour Diffusion After 1.81 Mgy X- Ray Dose At Esrf Id29 Beamline (best Case)
Crit. Rev. Eukaryot. Gene Expr. (2012) 22 p.219-232
A state-of-the-art review of the role of the Langmuir-Blodgett nanotemplate on protein crystal structures is here presented. Crystals grown by nanostructured template appear more radiation resistant than the classical ones, even in the presence of a third-generation highly focused beam at the European Synchrotron Radiation Facility. The electron density maps and the changes in parameters such as total diffractive power, B-factor, and pairwise R-factor have been discussed. Protein crystals, grown by the Langmuir-Blodgett nanotemplate-based method, proved to be more radiation resistant compared to crystals grown by the classical hanging drop method in terms of both global and specific damage.