3V3T: Crystal Structure of Clostridium Botulinum Phage C-st Tubz

Citation:
Abstract
Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 A structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.
PDB ID: 3V3TDownload
MMDB ID: 99684
PDB Deposition Date: 2011/12/14
Updated in MMDB: 2012/05 
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 3V3T: monomeric; determined by author and by software (PISA)
Molecular Components in 3V3T
Label Count Molecule
Protein (1 molecule)
1
Cell Division Gtpase Ftsz, Diverged
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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