3V0B: 3.9 Angstrom Crystal Structure Of Bont/ai In Complex With Ntnha

Citation:
Abstract
Botulinum neurotoxins (BoNTs) are highly poisonous substances that are also effective medicines. Accidental BoNT poisoning often occurs through ingestion of Clostridium botulinum-contaminated food. Here, we present the crystal structure of a BoNT in complex with a clostridial nontoxic nonhemagglutinin (NTNHA) protein at 2.7 angstroms. Biochemical and functional studies show that NTNHA provides large and multivalent binding interfaces to protect BoNT from gastrointestinal degradation. Moreover, the structure highlights key residues in BoNT that regulate complex assembly in a pH-dependent manner. Collectively, our findings define the molecular mechanisms by which NTNHA shields BoNT in the hostile gastrointestinal environment and releases it upon entry into the circulation. These results will assist in the design of small molecules for inhibiting oral BoNT intoxication and of delivery vehicles for oral administration of biologics.
PDB ID: 3V0BDownload
MMDB ID: 97984
PDB Deposition Date: 2011/12/7
Updated in MMDB: 2013/09
Experimental Method:
x-ray diffraction
Resolution: 3.9  Å
Source Organism:
Similar Structures:
Biological Unit for 3V0B: dimeric; determined by author and by software (PISA)
Molecular Components in 3V0B
Label Count Molecule
Proteins (2 molecules)
1
Bont/a
Molecule annotation
1
Ntnh
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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