3UYP: Crystal Structure Of The Dengue Virus Serotype 4 Envelope Protein Domain Iii In Complex With The Variable Domains Of Mab 4e11

Citation:
Abstract
The dengue virus (DENV) complex is composed of four distinct but serologically related flaviviruses, which together cause the present-day most important emerging viral disease. Although DENV infection induces lifelong immunity against viruses of the same serotype, the antibodies raised appear to contribute to severe disease in cases of heterotypic infections. Understanding the mechanisms of DENV neutralization by antibodies is, therefore, crucial for the design of vaccines that simultaneously protect against all four viruses. Here, we report a comparative, high-resolution crystallographic analysis of an "A-strand" murine monoclonal antibody, Mab 4E11, in complex with its target domain of the envelope protein from the four DENVs. Mab 4E11 is capable of neutralizing all four serotypes, and our study reveals the determinants of this cross-reactivity. The structures also highlight the mechanism by which A-strand Mabs disrupt the architecture of the mature virion, inducing premature fusion loop exposure and concomitant particle inactivation.
PDB ID: 3UYPDownload
MMDB ID: 97453
PDB Deposition Date: 2011/12/6
Updated in MMDB: 2012/04
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Dengue virus 4
Similar Structures:
Biological Unit for 3UYP: dimeric; determined by author
Molecular Components in 3UYP
Label Count Molecule
Proteins (2 molecules)
1
Anti-dengue MAB 4e11
Molecule annotation
1
Envelope Protein
Molecule annotation
Chemicals (8 molecules)
1
6
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.