3UOQ: Crystal Structure Of Release Factor Rf3 Trapped In The Gtp State On A Rotated Conformation Of The Ribosome (Without Viomycin)

Citation:
Abstract
The class II release factor RF3 is a GTPase related to elongation factor EF-G, which catalyzes release of class I release factors RF1 and RF2 from the ribosome after termination of protein synthesis. The 3.3 A crystal structure of the RF3.GDPNP.ribosome complex provides a high-resolution description of interactions and structural rearrangements that occur when binding of this translational GTPase induces large-scale rotational movements in the ribosome. RF3 induces a 7 degrees rotation of the body and 14 degrees rotation of the head of the 30S ribosomal subunit, and itself undergoes inter- and intradomain conformational rearrangements. We suggest that ordering of critical elements of switch loop I and the P loop, which help to form the GTPase catalytic site, are caused by interactions between the G domain of RF3 and the sarcin-ricin loop of 23S rRNA. The rotational movements in the ribosome induced by RF3, and its distinctly different binding orientation to the sarcin-ricin loop of 23S rRNA, raise interesting implications for the mechanism of action of EF-G in translocation.
MMDB ID: 99557
PDB Deposition Date: 2011/11/17
Updated in MMDB: 2012/08
Experimental Method:
x-ray diffraction
Resolution: 3.7  Å
Source Organism:
synthetic construct
Similar Structures:
Merged PDB IDs:3UOQ 3UOS
Asymmetric Unit for 3UOQ: 58-meric
Molecular Components in 3UOQ
Label Count Molecule
Proteins (54 molecules)
1
30S Ribosomal Protein S2
Molecule annotation
1
30S Ribosomal Protein S3
Molecule annotation
1
30S Ribosomal Protein S4
Molecule annotation
1
30S Ribosomal Protein S5
Molecule annotation
1
30S Ribosomal Protein S6
Molecule annotation
1
30S Ribosomal Protein S7
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9
Molecule annotation
1
30S Ribosomal Protein S10
Molecule annotation
1
30S Ribosomal Protein S11
Molecule annotation
1
30S Ribosomal Protein S12
Molecule annotation
1
30S Ribosomal Protein S13
Molecule annotation
1
30S Ribosomal Protein S14
Molecule annotation
1
30S Ribosomal Protein S15
Molecule annotation
1
30S Ribosomal Protein S16
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18
Molecule annotation
1
30S Ribosomal Protein S19
Molecule annotation
1
30S Ribosomal Protein S20
Molecule annotation
1
30S Ribosomal Protein S21
Molecule annotation
1
Peptide Chain Release Factor 3
Molecule annotation
1
50S Ribosomal Protein L27
Molecule annotation
1
50S Ribosomal Protein L28
Molecule annotation
1
50S Ribosomal Protein L29
Molecule annotation
1
50S Ribosomal Protein L30
Molecule annotation
1
50S Ribosomal Protein L32
Molecule annotation
1
50S Ribosomal Protein L33
Molecule annotation
1
50S Ribosomal Protein L34
Molecule annotation
1
50S Ribosomal Protein L35
Molecule annotation
1
50S Ribosomal Protein L36
Molecule annotation
1
50S Ribosomal Protein L2
Molecule annotation
1
50S Ribosomal Protein L3
Molecule annotation
1
50S Ribosomal Protein L4
Molecule annotation
1
50S Ribosomal Protein L5
Molecule annotation
1
50S Ribosomal Protein L6
Molecule annotation
1
50S Ribosomal Protein L10
Molecule annotation
1
50S Ribosomal Protein L11
Molecule annotation
4
50S Ribosomal Protein L7/l12
Molecule annotation
1
50S Ribosomal Protein L13
Molecule annotation
1
50S Ribosomal Protein L14
Molecule annotation
1
50S Ribosomal Protein L15
Molecule annotation
1
50S Ribosomal Protein L16
Molecule annotation
1
50S Ribosomal Protein L17
Molecule annotation
1
50S Ribosomal Protein L18
Molecule annotation
1
50S Ribosomal Protein L19
Molecule annotation
1
50S Ribosomal Protein L20
Molecule annotation
1
50S Ribosomal Protein L21
Molecule annotation
1
50S Ribosomal Protein L22
Molecule annotation
1
50S Ribosomal Protein L23
Molecule annotation
1
50S Ribosomal Protein L24
Molecule annotation
1
50S Ribosomal Protein L25
Molecule annotation
Nucleotides(4 molecules)
1
16S rRNA
Molecule annotation
1
Messenger RNA
Molecule annotation
1
23S rRNA
Molecule annotation
1
5S rRNA
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

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