3UO7: Crystal Structure Of Human Thymine Dna Glycosylase Bound To Substrate 5-carboxylcytosine

Human thymine DNA glycosylase (hTDG) efficiently excises 5-carboxylcytosine (5caC), a key oxidation product of 5-methylcytosine in genomic DNA, in a recently discovered cytosine demethylation pathway. We present here the crystal structures of the hTDG catalytic domain in complex with duplex DNA containing either 5caC or a fluorinated analog. These structures, together with biochemical and computational analyses, reveal that 5caC is specifically recognized in the active site of hTDG, supporting the role of TDG in mammalian 5-methylcytosine demethylation.
PDB ID: 3UO7Download
MMDB ID: 97302
PDB Deposition Date: 2011/11/16
Updated in MMDB: 2015/06
Experimental Method:
x-ray diffraction
Resolution: 3  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 3UO7: tetrameric; determined by software (PISA)
Molecular Components in 3UO7
Label Count Molecule
Proteins (2 molecules)
G/T Mismatch-specific Thymine DNA Glycosylase(Gene symbol: TDG)
Molecule annotation
Nucleotides(2 molecules)
5'-d(*cp*ap*gp*cp*tp*cp*tp*gp*tp*ap*cp*ap*tp*gp*ap*gp*cp*ap *gp*tp*gp*gp*a)-3'
Molecule annotation
5'-d(*cp*cp*ap*cp*tp*gp*cp*tp*cp*ap*(1cc) P*gp*tp*ap*cp*ap*gp*ap*gp*cp*tp*gp*t)-3'
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB