3U01: Crystal structure of onconase double mutant C30A/C75A at 1.12 A resolution

The structure of onconase C30A/C75A double mutant has been determined at 1.12A resolution. The structure has high structural homology to other onconase structures. The changes being results of mutation are relatively small, distributed asymmetrically around the two mutated positions, and they are observed not only in the mutation region but expanded to entire molecule. Different conformation of Lys31 side chain that influences the hydrogen bonding network around catalytic triad is probably responsible for lower catalytic efficiency of double mutant. The decrease in thermal stability observed for the onconase variant might be explained by a less dense packing as manifested by the increase of the molecular volume and the solvent accessible surface area.
PDB ID: 3U01Download
MMDB ID: 95921
PDB Deposition Date: 2011/9/28
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 1.12  Å
Source Organism:
Similar Structures:
Biological Unit for 3U01: monomeric; determined by author and by software (PISA)
Molecular Components in 3U01
Label Count Molecule
Protein (1 molecule)
Protein P-30
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB