3THK: Structure Of Sh3 Chimera With A Type Ii Ligand Linked To The Chain C- Terminal

Citation:
Abstract
A new chimeric protein, named WT-CIIA, was designed by connecting the proline-rich decapeptide PPPVPPYSAG to the C-terminus of the alpha-spectrin SH3 domain through a natural twelve-residue linker to obtain a single-chain model that would imitate intramolecular SH3-ligand interaction. The crystal structure of this fusion protein was determined at 1.7 A resolution. The asymmetric unit of the crystal contained two SH3 globules contacting with one PPPVPPY fragment located between them. The domains are related by the two-fold non-crystallographic axis and the ligand lies in two opposite orientations with respect to the conservative binding sites of SH3 domains.
PDB ID: 3THKDownload
MMDB ID: 95263
PDB Deposition Date: 2011/8/19
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3THK: dimeric; determined by author
Molecular Components in 3THK
Label Count Molecule
Proteins (2 molecules)
1
Spectrin Alpha Chain, Brain(Gene symbol: Sptan1)
Molecule annotation
1
Proline-rich Peptide
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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