3T97: Molecular Architecture Of The Transport Channel Of The Nuclear Pore Complex: Nup62NUP54

The nuclear pore complex encloses a central channel for nucleocytoplasmic transport, which is thought to consist of three nucleoporins, Nup54, Nup58, and Nup62. However, the structure and composition of the channel are elusive. We determined the crystal structures of the interacting domains between these nucleoporins and pieced together the molecular architecture of the mammalian transport channel. Located in the channel midplane is a flexible Nup54Nup58 ring that can undergo large rearrangements yielding diameter changes from approximately 20 to approximately 40 nm. Nup62Nup54 triple helices project alternately up and down from either side of the midplane ring and form nucleoplasmic and cytoplasmic entries. The channel consists of as many as 224 copies of the three nucleoporins, amounting to a molar mass of 12.3 MDa and contributing 256 phenylalanine-glycine repeat regions. We propose that the occupancy of these repeat regions with transport receptors modulates ring diameter and transport activity.
PDB ID: 3T97Download
MMDB ID: 94809
PDB Deposition Date: 2011/8/2
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Similar Structures:
Biological Unit for 3T97: trimeric; determined by author and by software (PISA)
Molecular Components in 3T97
Label Count Molecule
Proteins (3 molecules)
Nuclear Pore Complex Protein Nup54(Gene symbol: Nup54)
Molecule annotation
Nuclear Pore Glycoprotein P62(Gene symbol: Nup62)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB