3T94: Crystal Structure Of 5'-Deoxy-5'-Methylthioadenosine Phosphorylase (Mtap) Ii Complexed With 5'-Deoxy-5'-Methylthioadenosine And Sulfate

5'-Deoxy-5'-methylthioadenosine phosphorylase (MTAP) catalyzes the phosphorolytic cleavage of 5'-deoxy-5'-methylthioadenosine (MTA), a byproduct of polyamine biosynthesis. The Sulfolobus sulfataricus genome encodes two MTAPs. SsMTAP I has broad substrate specifity, accepting guanosine, inosine, adenosine and MTA, while SsMTAP II is specific for MTA. SsMTAP I forms a donut-shaped hexamer, while SsMTAP II is a hexamer formed from trimers packed face to face. The structure of SsMTAP II was originally determined in space group P1 (PDB entry 2a8y) and showed R32 pseudosymmetry. Post-analysis using phenix.xtriage showed that the correct space group is C2. Here, the structure refined in space group C2 is reported and the factors that initially led to the incorrect space-group assignment are discussed.
PDB ID: 3T94Download
MMDB ID: 92986
PDB Deposition Date: 2011/8/2
Updated in MMDB: 2011/08
Experimental Method:
x-ray diffraction
Resolution: 1.45  Å
Source Organism:
Similar Structures:
Biological Unit for 3T94: hexameric; determined by author and by software (PISA)
Molecular Components in 3T94
Label Count Molecule
Proteins (6 molecules)
5'-methylthioadenosine Phosphorylase (Mtap)(Gene symbol: SSO_RS11395)
Molecule annotation
Chemicals (17 molecules)
* Click molecule labels to explore molecular sequence information.

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