3T4B: Crystal Structure Of The Hcv Ires Pseudoknot Domain

Translation of hepatitis C viral proteins requires an internal ribosome entry site (IRES) located in the 5' untranslated region of the viral mRNA. The core domain of the hepatitis C virus (HCV) IRES contains a four-way helical junction that is integrated within a predicted pseudoknot. This domain is required for positioning the mRNA start codon correctly on the 40S ribosomal subunit during translation initiation. Here, we present the crystal structure of this RNA, revealing a complex double-pseudoknot fold that establishes the alignment of two helical elements on either side of the four-helix junction. The conformation of this core domain constrains the open reading frame's orientation for positioning on the 40S ribosomal subunit. This structure, representing the last major domain of HCV-like IRESs to be determined at near-atomic resolution, provides the basis for a comprehensive cryoelectron microscopy-guided model of the intact HCV IRES and its interaction with 40S ribosomal subunits.
PDB ID: 3T4BDownload
MMDB ID: 94282
PDB Deposition Date: 2011/7/25
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 3.55  Å
Biological Unit for 3T4B: monomeric; determined by author
Molecular Components in 3T4B
Label Count Molecule
Nucleotide(1 molecule)
HCV Ires Pseudoknot Domain Plus Crystallization Module
Molecule annotation
Chemicals (17 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB