3SZI: Structure Of Apo Shwanavidin (P21 Form)

Citation:
Abstract
Shwanavidin is an avidin-like protein from the marine proteobactrium Shewanella denitrificans, which exhibits an innate dimeric structure while maintaining high affinity toward biotin. A unique residue (Phe-43) from the L3,4 loop and a distinctive disulfide bridge were shown to account for the high affinity toward biotin. Phe-43 emulates the function and position of the critical intermonomeric Trp that characterizes the tetrameric avidins but is lacking in shwanavidin. The 18 copies of the apo-monomer revealed distinctive snapshots of L3,4 and Phe-43, providing rare insight into loop flexibility, binding site accessibility, and psychrophilic adaptation. Nevertheless, as in all avidins, shwanavidin also displays high thermostability properties. The unique features of shwanavidin may provide a platform for the design of a long sought after monovalent form of avidin, which would be ideal for novel types of biotechnological application.
PDB ID: 3SZIDownload
MMDB ID: 98976
PDB Deposition Date: 2011/7/19
Updated in MMDB: 2012/06
Experimental Method:
x-ray diffraction
Resolution: 1.4  Å
Source Organism:
Similar Structures:
Biological Unit for 3SZI: dimeric; determined by author and by software (PISA)
Molecular Components in 3SZI
Label Count Molecule
Proteins (2 molecules)
2
Avidinstreptavidin
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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