3SVM: Human Mpp8 - Human Dnmt3ak47me2 Peptide

DNA CpG methylation and histone H3 lysine 9 (H3K9) methylation are two major repressive epigenetic modifications, and these methylations are positively correlated with one another in chromatin. Here we show that G9a or G9a-like protein (GLP) dimethylate the amino-terminal lysine 44 (K44) of mouse Dnmt3a (equivalent to K47 of human DNMT3A) in vitro and in cells overexpressing G9a or GLP. The chromodomain of MPP8 recognizes the dimethylated Dnmt3aK44me2. MPP8 also interacts with self-methylated GLP in a methylation-dependent manner. The MPP8 chromodomain forms a dimer in solution and in crystals, suggesting that a dimeric MPP8 molecule could bridge the methylated Dnmt3a and GLP, resulting in a silencing complex of Dnmt3a-MPP8-GLP/G9a on chromatin templates. Together, these findings provide a molecular explanation, at least in part, for the co-occurrence of DNA methylation and H3K9 methylation in chromatin.
PDB ID: 3SVMDownload
MMDB ID: 95372
PDB Deposition Date: 2011/7/12
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 2.31  Å
Source Organism:
Similar Structures:
Biological Unit for 3SVM: dimeric; determined by author and by software (PISA)
Molecular Components in 3SVM
Label Count Molecule
Proteins (2 molecules)
M-phase Phosphoprotein 8(Gene symbol: MPHOSPH8)
Molecule annotation
DNA (Cytosine-5)-methyltransferase 3A(Gene symbol: DNMT3A)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB