3SPE: Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ

Citation:
Abstract
Bacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. The phiKZ virion consists of a 1450 A diameter icosahedral head and a 2000 A-long contractile tail. The structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied Myovirus bacteriophage T4 tail. The crystal structure of a tail sheath protein fragment of phiKZ was determined to 2.4 A resolution. Furthermore, crystal structures of two prophage tail sheath proteins were determined to 1.9 and 3.3 A resolution. Despite low sequence identity between these proteins, all of these structures have a similar fold. The crystal structure of the phiKZ tail sheath protein has been fitted into cryo-electron-microscopy reconstructions of the extended tail sheath and of a polysheath. The structural rearrangement of the phiKZ tail sheath contraction was found to be similar to that of phage T4.
PDB ID: 3SPEDownload
MMDB ID: 95732
PDB Deposition Date: 2011/7/1
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 2.3996  Å
Source Organism:
Similar Structures:
Biological Unit for 3SPE: monomeric; determined by author
Molecular Components in 3SPE
Label Count Molecule
Protein (1 molecule)
1
Phikz029
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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