3SGD: Crystal Structure Of The Mouse Mab 17.2

Citation:
Abstract
Patients with Chronic Chagas' Heart Disease possess high levels of antibodies against the carboxyl-terminal end of the ribosomal P2ss protein of Trypanosoma cruzi (TcP2ss). These antibodies, as well as the murine monoclonal antibody (mAb) 17.2, recognize the last 13 amino acids of TcP2ss (called the R13 epitope: EEEDDDMGFGLFD) and are able to cross-react with, and stimulate, the ss1 adrenergic receptor (ss1-AR). Indeed, the mAb 17.2 was able to specifically detect human beta1-AR, stably transfected into HEK cells, by flow cytometry and to induce repolarisation abnormalities and first degree atrioventricular conduction block after passive transfer to naive mice. To study the structural basis of this cross-reactivity, we determined the crystal structure of the Fab region of the mAb 17.2 alone at 2.31 A resolution and in complex with the R13 peptide at 1.89 A resolution. We identified as key contact residues on R13 peptide Glu3, Asp6 and Phe9 as was previously shown by alanine scanning. Additionally, we generated a model of human beta1-AR to elucidate the interaction with anti-R13 antibodies. These data provide an understanding of the molecular basis of cross-reactive antibodies induced by chronic infection with Trypanosoma cruzi.
PDB ID: 3SGDDownload
MMDB ID: 94906
PDB Deposition Date: 2011/6/14
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 2.31  Å
Source Organism:
Similar Structures:
Biological Unit for 3SGD: dimeric; determined by author and by software (PISA)
Molecular Components in 3SGD
Label Count Molecule
Proteins (2 molecules)
1
Light Chain
Molecule annotation
1
Heavy Chain
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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