3RYH: Gmpcpp-Tubulin: Rb3 Stathmin-Like Domain Complex

Citation:
Abstract
Tubulin alternates between a soluble curved structure and a microtubule straight conformation. GTP binding to alphabeta-tubulin is required for microtubule assembly, but whether this triggers conversion into a straighter structure is still debated. This is due, at least in part, to the lack of structural data for GTP-tubulin before assembly. Here, we report atomic-resolution crystal structures of soluble tubulin in the GDP and GTP nucleotide states in a complex with a stathmin-like domain. The structures differ locally in the neighborhood of the nucleotide. A loop movement in GTP-bound tubulin favors its recruitment to the ends of growing microtubules and facilitates its curved-to-straight transition, but this conversion has not proceeded yet. The data therefore argue for the conformational change toward the straight structure occurring as microtubule-specific contacts are established. They also suggest a model for the way the tubulin structure is modified in relation to microtubule assembly.
PDB ID: 3RYHDownload
MMDB ID: 94049
PDB Deposition Date: 2011/5/11
Updated in MMDB: 2011/10
Experimental Method:
x-ray diffraction
Resolution: 2.8  Å
Source Organism:
Rattus norvegicus
Similar Structures:
Biological Unit for 3RYH: pentameric; determined by author and by software (PISA)
Molecular Components in 3RYH
Label Count Molecule
Proteins (5 molecules)
2
Tubulin Alpha Chain
Molecule annotation
2
Tubulin Beta Chain
Molecule annotation
1
Stathmin-4(Gene symbol: Stmn4)
Molecule annotation
Chemicals (16 molecules)
1
2
2
4
3
8
4
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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