3RPT: Crystal structure of the anti-HIV b12 scaffold protein

The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.
PDB ID: 3RPTDownload
MMDB ID: 95894
PDB Deposition Date: 2011/4/27
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 1.303  Å
Source Organism:
Similar Structures:
Biological Unit for 3RPT: monomeric; determined by author
Molecular Components in 3RPT
Label Count Molecule
Protein (1 molecule)
Endoglucanase E-2
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB