3RL4: Rat metallophosphodiesterase MPPED2 G252H Mutant

Citation:
Abstract
Metallophosphoesterase-domain-containing protein 2 (MPPED2) is a highly evolutionarily conserved protein with orthologs found from worms to humans. The human MPPED2 gene is found in a region of chromosome 11 that is deleted in patients with WAGR (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) syndrome, and MPPED2 may function as a tumor suppressor. However, the precise cellular roles of MPPED2 are unknown, and its low phosphodiesterase activity suggests that substrate hydrolysis may not be its prime function. We present here the structures of MPPED2 and two mutants, which show that the poor activity of MPPED2 is not only a consequence of the substitution of an active-site histidine residue by glycine but also due to binding of AMP or GMP to the active site. This feature, enhanced by structural elements of the protein, allows MPPED2 to utilize the conserved phosphoprotein-phosphatase-like fold in a unique manner, ensuring that its enzymatic activity can be combined with a possible role as a scaffolding or adaptor protein.
PDB ID: 3RL4Download
MMDB ID: 92558
PDB Deposition Date: 2011/4/19
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 1.29  Å
Source Organism:
Similar Structures:
Biological Unit for 3RL4: monomeric; determined by author and by software (PISA)
Molecular Components in 3RL4
Label Count Molecule
Protein (1 molecule)
1
Metallophosphoesterase Mpped2(Gene symbol: Mpped2)
Molecule annotation
Chemicals (11 molecules)
1
8
2
1
3
1
4
1
* Click molecule labels to explore molecular sequence information.

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