3RB5: Crystal structure of calcium binding domain CBD12 of CALX1.1

Citation:
Abstract
The Na(+)/Ca(2+) exchanger CALX promotes Ca(2+) efflux in Drosophila sensory neuronal cells to facilitate light-mediated Ca(2+) homeostasis. CALX activity is negatively regulated by specific Ca(2+) interaction within its two intracellular Ca(2+) regulatory domains CBD1 and CBD2, yet how the Ca(2+) binding is converted to molecular motion to operate the exchanger is unknown. Here, we report crystal structures of the entire Ca(2+) regulatory domain CBD12 from two alternative splicing isoforms, CALX 1.1 and 1.2, exhibiting distinct regulatory Ca(2+) dependency. The structures show an open V-shaped conformation with four Ca(2+) ions bound on the CBD domain interface, confirmed by LRET analysis. The structures together with Ca(2+)-binding analysis support that the Ca(2+) inhibition of CALX is achieved by interdomain conformational changes induced by Ca(2+) binding at CBD1. The conformational difference between the two isoforms also indicates that alternative splicing adjusts the interdomain orientation angle to modify the Ca(2+) regulatory property of the exchangers.
PDB ID: 3RB5Download
MMDB ID: 94778
PDB Deposition Date: 2011/3/28
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.35  Å
Source Organism:
Similar Structures:
Biological Unit for 3RB5: monomeric; determined by author
Molecular Components in 3RB5
Label Count Molecule
Protein (1 molecule)
1
Na/ca Exchange Protein
Molecule annotation
Chemicals (8 molecules)
1
4
2
1
3
3
* Click molecule labels to explore molecular sequence information.

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