3QT9: Analysis Of A New Family Of Widely Distributed Metal-Independent Alpha Mannosidases Provides Unique Insight Into The Processing Of N-Linked Glycans, Clostridium Perfringens Cpe0426 Complexed With Alpha-1,6- Linked 1-Thio-Alpha-Mannobiose

Citation:
Abstract
The modification of N-glycans by alpha-mannosidases is a process that is relevant to a large number of biologically important processes, including infection by microbial pathogens and colonization by microbial symbionts. At present, the described mannosidases specific for alpha1,6-mannose linkages are very limited in number. Through structural and functional analysis of two sequence-related enzymes, one from Streptococcus pneumoniae (SpGH125) and one from Clostridium perfringens (CpGH125), a new glycoside hydrolase family, GH125, is identified and characterized. Analysis of SpGH125 and CpGH125 reveal them to have exo-alpha1,6-mannosidase activity consistent with specificity for N-linked glycans having their alpha1,3-mannose branches removed. The x-ray crystal structures of SpGH125 and CpGH125 obtained in apo-, inhibitor-bound, and substrate-bound forms provide both mechanistic and molecular insight into how these proteins, which adopt an (alpha/alpha)(6)-fold, recognize and hydrolyze the alpha1,6-mannosidic bond by an inverting, metal-independent catalytic mechanism. A phylogenetic analysis of GH125 proteins reveals this to be a relatively large and widespread family found frequently in bacterial pathogens, bacterial human gut symbionts, and a variety of fungi. Based on these studies we predict this family of enzymes will primarily comprise such exo-alpha1,6-mannosidases.
PDB ID: 3QT9Download
MMDB ID: 89127
PDB Deposition Date: 2011/2/22
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 2.05  Å
Source Organism:
Similar Structures:
Biological Unit for 3QT9: monomeric; determined by author and by software (PISA)
Molecular Components in 3QT9
Label Count Molecule
Protein (1 molecule)
1
Putative Uncharacterized Protein Cpe0426
Molecule annotation
Chemicals (7 molecules)
1
6
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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