3Q0Q: Crystal Structure Of The Pumilio-homology Domain From Human Pumilio2 In Complex With P38alpha Nrea

Citation:
Abstract
Human PUMILIO1 (PUM1) and PUMILIO2 (PUM2) are members of the PUMILIO/FBF (PUF) family that regulate specific target mRNAs posttranscriptionally. Recent studies have identified mRNA targets associated with human PUM1 and PUM2. Here, we explore the structural basis of natural target RNA recognition by human PUF proteins through crystal structures of the RNA-binding domains of PUM1 and PUM2 in complex with four cognate RNA sequences, including sequences from p38alpha and erk2 MAP kinase mRNAs. We observe three distinct modes of RNA binding around the fifth RNA base, two of which are different from the prototypical 1 repeat:1 RNA base binding mode previously identified with model RNA sequences. RNA-binding affinities of PUM1 and PUM2 are not affected dramatically by the different binding modes in vitro. However, these modes of binding create structurally variable recognition surfaces that suggest a mechanism in vivo for recruitment of downstream effector proteins defined by the PUF:RNA complex.
PDB ID: 3Q0QDownload
MMDB ID: 89255
PDB Deposition Date: 2010/12/15
Updated in MMDB: 2013/06
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 3Q0Q: dimeric; determined by author and by software (PISA)
Molecular Components in 3Q0Q
Label Count Molecule
Protein (1 molecule)
1
Pumilio Homolog 2(Gene symbol: PUM2)
Molecule annotation
Nucleotide(1 molecule)
1
5'-r(up*gp*up*ap*ap*ap*up*a)-3'
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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